User:Moira M. Esson/Notebook/CHEM-581/2013/02/20: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(5 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 39: | Line 39: | ||
General Protocol: | General Protocol: | ||
# A 9 in. Corning disposable, non-sterile Pasteur pipette was heated using a Bunsen burner and was bent in various ways(multiple bends at the stem of the pipette and the top, bends only at the top, etc.). Note: Pictures of bent pipettes will be uploaded for reference. | # A 9 in. Corning disposable, non-sterile Pasteur pipette was heated using a Bunsen burner and was bent in various ways(multiple bends at the stem of the pipette and the top, bends only at the top, etc.). Note: Pictures of bent pipettes will be uploaded for reference. | ||
# Using a razor blade, the hydrogel for testing was cut into small cubes. The mass of each tested sample should be approximately 0.1g. | # Using a razor blade, the hydrogel for testing was cut into small cubes. The mass of each tested sample should be approximately 0.1g. If the hydrogel was too firm for safe cutting, the hydrogel was placed in 3mL distilled H<sub>2</sub>O for approximately 20 minutes. The time in distilled H<sub>2</sub>O can not exceed 2 hours(definitive amount of time that was determined where minimal leakage of dye occurred). | ||
# 3mL of distilled H<sub>2</sub>O were added to the pipette and using a ribber bulb squeezed out of the pipette. The 3mL of H<sub>2</sub>O were collected in a beaker. | # 3mL of distilled H<sub>2</sub>O were added to the pipette and using a ribber bulb squeezed out of the pipette. The 3mL of H<sub>2</sub>O were collected in a beaker. Note: When adding the distilled H<sub>2</sub>O, the tip of the pipette should be completely covered with a finger or a hand to prevent any H<sub>2</sub>O from going straight through the pipette. | ||
# Fluorescence was then run on the collected sample. | # Fluorescence was then run on the collected sample. | ||
<br> | <br> | ||
Line 52: | Line 52: | ||
| || | | || | ||
|- | |- | ||
| Shape of pasteur pipette||Amount of hydrogel used(g) | | '''Shape of pasteur pipette'''||'''Amount of hydrogel used'''(g) | ||
|- | |- | ||
| Plain pasteur pipette. No modifications||0.1575 | | Plain pasteur pipette. No modifications||0.1575 | ||
Line 77: | Line 77: | ||
|} | |} | ||
<br> | <br> | ||
Figure 1. Spectra for the pressure testing of 90:10 PVA MW 146,000-186,000:110% Lamponite | |||
<br> | |||
[[Image:Fluorescence spectra determining the best pipette for pressure testing.png]] | |||
<br> | |||
*It appears as if using an unmodified pipette or a pipette with a bend at the top of the pipette so the sides are almost touching produced the maximum amount of sheer pressure. Further testing will be done for verification. | *It appears as if using an unmodified pipette or a pipette with a bend at the top of the pipette so the sides are almost touching produced the maximum amount of sheer pressure. Further testing will be done for verification. | ||
*In comparison to the 0.25μM Rhodamine 6G control, this method of sheer pressure does not perform a significant amount of sheer pressure or does not elicit the diffusion of a significant amount of dye. Another method to test the sheer pressure will be considered. | |||
Latest revision as of 22:29, 26 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||
Objectives
Microsphere preparation
Hydrogel Pressure testing
|