User:Moira M. Esson/Notebook/CHEM-581/2013/02/20: Difference between revisions
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General Protocol: | General Protocol: | ||
# A 9 in. Corning disposable, non-sterile Pasteur pipette was heated using a Bunsen burner and was bent in various ways(multiple bends at the stem of the pipette and the top, bends only at the top, etc.). Note: Pictures of bent pipettes will be uploaded for reference. | # A 9 in. Corning disposable, non-sterile Pasteur pipette was heated using a Bunsen burner and was bent in various ways(multiple bends at the stem of the pipette and the top, bends only at the top, etc.). Note: Pictures of bent pipettes will be uploaded for reference. | ||
# Using a razor blade, the hydrogel for testing was cut into small cubes. The mass of each tested sample should be approximately 0.1g. | # Using a razor blade, the hydrogel for testing was cut into small cubes. The mass of each tested sample should be approximately 0.1g. If the hydrogel was too firm for safe cutting, the hydrogel was placed in 3mL distilled H<sub>2</sub>O for approximately 20 minutes. The time in distilled H<sub>2</sub>O can not exceed 2 hours(definitive amount of time that was determined where minimal leakage of dye occurred). | ||
# 3mL of distilled H<sub>2</sub>O were added to the pipette and using a ribber bulb squeezed out of the pipette. The 3mL of H<sub>2</sub>O were collected in a beaker. | # 3mL of distilled H<sub>2</sub>O were added to the pipette and using a ribber bulb squeezed out of the pipette. The 3mL of H<sub>2</sub>O were collected in a beaker. Note: When adding the distilled H<sub>2</sub>O, the tip of the pipette should be completely covered with a finger or a hand to prevent any H<sub>2</sub>O from going straight through the pipette. | ||
# Fluorescence was then run on the collected sample. | # Fluorescence was then run on the collected sample. | ||
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Revision as of 20:21, 2 March 2013
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Objectives
Microsphere preparation
Hydrogel Pressure testing
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