User:Moira M. Esson/Notebook/CHEM-581/2013/02/20: Difference between revisions
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==Hydrogel Pressure testing== | ==Hydrogel Pressure testing== | ||
* After performing diffusion tests on all prepared hydrogels on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/15|2013/02/15]], it was determined that the hydrogels prepared with DMSO and dye added prior to the freeze thaw method did not leak dye at a fast rate. As such, pressure tests will be performed using these hydrogels because all collected dye will be caused solely by sheer pressure. | |||
*First, the most effective pipette for pressure testing was determined. | |||
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General Protocol: | |||
# A 9 in. Corning disposable, non-sterile Pasteur pipette was heated using a Bunsen burner and was bent in various ways(multiple bends at the stem of the pipette and the top, bends only at the top, etc.). Note: Pictures of bent pipettes will be uploaded for reference. | |||
# Using a razor blade, the hydrogel for testing was cut into small cubes. The mass of each tested sample should be approximately 0.1g. If the hydrogel was too firm for safe cutting, the hydrogel was placed in 3mL distilled H<sub>2</sub>O for approximately 20 minutes. The time in distilled H<sub>2</sub>O can not exceed 2 hours(definitive amount of time that was determined where minimal leakage of dye occurred). | |||
# 3mL of distilled H<sub>2</sub>O were added to the pipette and using a ribber bulb squeezed out of the pipette. The 3mL of H<sub>2</sub>O were collected in a beaker. Note: When adding the distilled H<sub>2</sub>O, the tip of the pipette should be completely covered with a finger or a hand to prevent any H<sub>2</sub>O from going straight through the pipette. | |||
# Fluorescence was then run on the collected sample. | |||
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*The same hydrogel sample will be used to test the various Pasteur pipettes. As such, the sample with the largest amount of fluorescence will indicate the most effective manner in which to bend the Pasteur pipette to create the most sheer pressure. | |||
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Pressure samples: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''90:10 PVA MW 146,000-186,000:110% Lamponite hydrogel pressure tests''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
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| '''Shape of pasteur pipette'''||'''Amount of hydrogel used'''(g) | |||
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| Plain pasteur pipette. No modifications||0.1575 | |||
|- | |||
| Stem of pipette bent at angle slightly less than 90 degrees||0.1134 | |||
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| Stem of pipette bent at angle slightly less than 90 degrees right at the start of the stem of pipette||0.1397 | |||
|- | |||
| Pipette with three pockets at the top of the pipette||0.1039 | |||
|- | |||
| Pipette bent twice in the stem||0.1158 | |||
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| Pipette bent at the top of the pipette at close to 90 degree angle. Sides of pipette almost touching||0.1108 | |||
|- | |||
| Pipette with a twist in the middle of the pipette||0.0993 | |||
|- | |||
| Pipette bent twice in the middle of the pipette||0.1416 | |||
|- | |||
| Pipette with two pockets in the top portion of the pipette||0.1041 | |||
|- | |||
| Top bent so sides almost touching at 90 degree angle||0.1258 | |||
|- | |||
| Plain pasteur pipette. No modifications. Run #2||0.121 | |||
|} | |||
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Figure 1. Spectra for the pressure testing of 90:10 PVA MW 146,000-186,000:110% Lamponite | |||
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[[Image:Fluorescence spectra determining the best pipette for pressure testing.png]] | |||
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*It appears as if using an unmodified pipette or a pipette with a bend at the top of the pipette so the sides are almost touching produced the maximum amount of sheer pressure. Further testing will be done for verification. | |||
*In comparison to the 0.25μM Rhodamine 6G control, this method of sheer pressure does not perform a significant amount of sheer pressure or does not elicit the diffusion of a significant amount of dye. Another method to test the sheer pressure will be considered. | |||
Revision as of 22:01, 6 March 2013
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Objectives
Microsphere preparation
Hydrogel Pressure testing
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