User:Moira M. Esson/Notebook/CHEM-581/2013/03/01

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Objectives

  1. Conduct pressure tests on all hydrogels using an unmodified pipette.
  2. Run fluorescence on all samples collected from the pressure tests.
  3. Decant the safflower oil from the microsphere samples prepared on 2013/02/22.


Pressure tests using an unmodified pipette

  • All pressure tests were conducted following the general protocol described on 2013/02/20.
  • Pressure tests were conducted using an unmodified pipette in order to determine if the very slight amount of sheer pressure caused by an unmodified pipette would cause a detectable amount of dye diffusion.


Table 1. Information concerning the pressure testing involving hydrogels with an unmodified pipette.

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
150:50130K110% CEC NaMT w/ DMHXLBR0.1006
250:50130K110% CEC NaMT w/ DMHXLBR0.1022
390:10130KLaponite0.1031
450:50146K110% CEC Laponite w/ DMHXLBR0.1066
590:10130K110% CEC Laponite w/ DMHXLBR0.1083
690:10130K110% CEC NaMT w/ DMHXLBR0.1003
750:50130K110% CEC Laponite w/ DMHXLBR0.1036
850:50146KLaponite0.1016
990:10130K110% CEC NaMT w/ DMHXLBR0.1011
1090:10146K110% CEC NaMT w/ DMHXLBR0.1020
1150:50130KNaMT0.1024
1250:50130K50% CEC NaMT w/ Bu3HdP+0.1015
1350:50146K110% CEC NaMT w/ DMHXLBR0.0998
1490:10130K50% CEC NaMT w/ Bu3HdP+0.1026


Figure 1. Fluorescence of hydrogel samples modified with Lamponite clay using an unmodified pipette for pressure testing
Figure 2. Fluorescence of hydrogel samples modified with NaMT clay using an unmodified pipette for pressure testing
Figure 3. Fluorescence of all hydrogel samples using and unmodified pipette for pressure testing
Notes:

  • The amount of dye, or concentration of dye, that was diffused from the hydrogels using an unmodified pipette was significantly lower than that of a bent pipette.
  • The general trends observed using a bent pipette were also observed with an unmodified pipette.


Microspheres

  • After the freeze



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