User:Moira M. Esson/Notebook/CHEM-581/2013/03/20: Difference between revisions
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==Microsphere Dye Addition== | ==Microsphere Dye Addition== | ||
*The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/ | *The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/08|2013/02/08]] was followed. | ||
*An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels | *An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels | ||
*Dye was added to all the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/ | *Dye was added to all the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] | ||
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Dilution calculations: | Dilution calculations: | ||
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==Microsphere preparation== | ==Microsphere preparation== | ||
*The general protocol for microsphere preparation described on | *The general protocol for microsphere preparation described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] and [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/22|2013/02/22]]. | ||
*The following preparations were run on a 0.1g scale as opposed to a 1g scale in the hopes of preventing the formation of hydrogels. | |||
*A very small amount of PVA/clay was added to each vial and the vial was filled with safflower oil prior to the rapid freezing in order to achieve a true emulsion of microspheres in the organic safflower oil. This may prevent the formation of hydrogels. | |||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio''' | | align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio''' | ||
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| 90:10||110% CEC NaMT||0.01070||0.09100||20||35 | | 90:10||110% CEC NaMT||0.01070||0.09100||20||35 | ||
|} | |} | ||
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==Notes== | |||
*Dye was added to the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] prior to a DSC analysis. These samples will need to be reprepared for DSC analysis. | |||
Revision as of 17:56, 24 March 2013
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Objectives
Microsphere Dye Addition
M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Microsphere preparation
Notes
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