User:Moira M. Esson/Notebook/CHEM-581/2013/03/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objectives== | ||
* | # Finish rinsing and filtering prepared microspheres with hexanes. | ||
# Continue microsphere preparation. | |||
# Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive. | |||
<br> | |||
==Microsphere Dye Addition== | |||
*The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/08|2013/02/08]] was followed. | |||
*An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels | |||
*Dye was added to all the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] | |||
<br> | |||
Dilution calculations: | |||
<br> | |||
'''1μM Rhodamine 6G Dye Concentration (90:10)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 109μL | |||
'''1μM Rhodamine 6G Dye Concentration (50:50)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 61μL | |||
<br> | |||
==Microsphere preparation== | |||
*The general protocol for microsphere preparation described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] and [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/22|2013/02/22]]. | |||
*The following preparations were run on a 0.1g scale as opposed to a 1g scale in the hopes of preventing the formation of hydrogels. | |||
*A very small amount of PVA/clay was added to each vial and the vial was filled with safflower oil prior to the rapid freezing in order to achieve a true emulsion of microspheres in the organic safflower oil. This may prevent the formation of hydrogels. | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Clay Selection''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Safflower oil Added (mL)''' | |||
|- | |||
| 50:50||110% CEC NaMT||0.05010||0.05100||20||35 | |||
|- | |||
| 90:10||110% CEC NaMT||0.01070||0.09100||20||35 | |||
|} | |||
<br> | |||
==Notes== | |||
*Dye was added to the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] prior to a DSC analysis. These samples will need to be reprepared for DSC analysis. | |||
Latest revision as of 22:34, 26 September 2017
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Objectives
Microsphere Dye Addition
M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Microsphere preparation
Notes
|