User:Moira M. Esson/Notebook/CHEM-581/2013/03/20: Difference between revisions

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==Microsphere Dye Addition==
==Microsphere Dye Addition==
*The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/20]] was followed.
*The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/15|2013/02/15]] was followed.
*An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
*An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
*Dye was added to all the microspheres prepared on [[User:Moira_M.Esson/Notebook/CHEM-581/2013/02/22|2013/02/22]]
*Dye was added to all the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/22]]
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Dilution calculations:
Dilution calculations:
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   1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
   1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
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==Microsphere preparation==
==Microsphere preparation==
*The general protocol for microsphere preparation described on  
*The general protocol for microsphere preparation described on  

Revision as of 17:47, 24 March 2013

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Objectives

  1. Finish rinsing and filtering prepared microspheres with hexanes.
  2. Continue microsphere preparation.
  3. Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive.


Microsphere Dye Addition

  • The general protocol for dye addition to PVA hydrogel/microspheres described on 2013/02/15 was followed.
  • An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
  • Dye was added to all the microspheres prepared on 2013/02/22


Dilution calculations:
1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL


Microsphere preparation

  • The general protocol for microsphere preparation described on
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Safflower oil Added (mL)
50:50 110% CEC NaMT 0.05010 0.05100 20 35
90:10 110% CEC NaMT 0.01070 0.09100 20 35



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