User:Moira M. Esson/Notebook/CHEM-581/2013/03/20: Difference between revisions
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==Microsphere Dye Addition== | ==Microsphere Dye Addition== | ||
*The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/ | *The general protocol for dye addition to PVA hydrogel/microspheres described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/15|2013/02/15]] was followed. | ||
*An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels | *An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels | ||
*Dye was added to all the microspheres prepared on [[User:Moira_M. | *Dye was added to all the microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/20|2013/02/22]] | ||
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Dilution calculations: | Dilution calculations: | ||
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1μM (RG6)x 10mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 61μL | 1μM (RG6)x 10mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 61μL | ||
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==Microsphere preparation== | ==Microsphere preparation== | ||
*The general protocol for microsphere preparation described on | *The general protocol for microsphere preparation described on |
Revision as of 17:47, 24 March 2013
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Objectives
Microsphere Dye Addition
M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Microsphere preparation
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