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Objectives
- Finish rinsing and filtering prepared microspheres with hexanes.
- Continue microsphere preparation.
- Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive.
Microsphere Dye Addition
- The general protocol for dye addition to PVA hydrogel/microspheres described on 2013/02/20 was followed.
- An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
Dilution calculations:
1μM Rhodamine 6G Dye Concentration (90:10)
M1V1 = M2V2
1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL
1μM Rhodamine 6G Dye Concentration (50:50)
M1V1 = M2V2
1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Dye Added to the Microspheres:
| PVOH vs. Clay Ratio
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Clay Selection
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PVOH 146K Mass (g)
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Actual Clay Mass (g)
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H2O Added (mL)
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Dye Concentration (μM)
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Dye Amount Added (μL)
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| 50:50 |
110% CEC NaMT |
0.05010 |
0.05100 |
40 |
165 |
61
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| 90:10 |
110% CEC NaMT |
0.01070 |
0.09100 |
40 |
92 |
109
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