The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
Objectives
- Finish rinsing and filtering prepared microspheres with hexanes.
- Continue microsphere preparation.
- Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive.
Microsphere Dye Addition
- The general protocol for dye addition to PVA hydrogel/microspheres described on 2013/02/20 was followed.
- An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
- Dye was added to all the microspheres prepared on
Dilution calculations:
1μM Rhodamine 6G Dye Concentration (90:10)
M1V1 = M2V2
1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL
1μM Rhodamine 6G Dye Concentration (50:50)
M1V1 = M2V2
1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Dye Added to the Microspheres:
PVOH vs. Clay Ratio
|
Clay Selection
|
PVOH 146K Mass (g)
|
Actual Clay Mass (g)
|
H2O Added (mL)
|
Dye Concentration (μM)
|
Dye Amount Added (μL)
|
50:50 |
110% CEC NaMT |
0.05010 |
0.05100 |
40 |
165 |
61
|
90:10 |
110% CEC NaMT |
0.01070 |
0.09100 |
40 |
92 |
109
|
|