User:Moira M. Esson/Notebook/CHEM-581/2013/04/03: Difference between revisions
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A new general protocol for the preparation of was used for the diffusion testing of the prepared microspheres. It was determined that the previous protocol did not allow for a quantitative understanding of the amount of dye diffused from the samples. | A new general protocol for the preparation of was used for the diffusion testing of the prepared microspheres. It was determined that the previous protocol did not allow for a quantitative understanding of the amount of dye diffused from the samples. | ||
<br> | <br> | ||
General Protocol: | '''General Protocol''': | ||
# The microspheres were removed from their respective vial and placed in new, clean 20mL vials. | # The microspheres were removed from their respective vial and placed in new, clean 20mL vials. | ||
# 15mL of deionized H<sub>2</sub>O were added to each vial. | # 15mL of deionized H<sub>2</sub>O were added to each vial. | ||
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# Once a spectrum was obtained, the fluorescence sample was replaced in the diffusion vial. | # Once a spectrum was obtained, the fluorescence sample was replaced in the diffusion vial. | ||
# This was repeated for 2 hours. | # This was repeated for 2 hours. | ||
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'''General information on the parameters of the fluorescence run''': | |||
#Starting wavelength: 500nm | |||
#End wavelength: 650nm | |||
#Excitation wavelength: 526nm | |||
#Exitation slit: 10 | |||
#Emission slit: 10 | |||
#Scan rate/Speed: 1200 | |||
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Revision as of 07:40, 5 April 2013
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Objectives
DecantingThe general protocol described on 2013/03/01 was followed.
DSC
Diffusion TestingA new general protocol for the preparation of was used for the diffusion testing of the prepared microspheres. It was determined that the previous protocol did not allow for a quantitative understanding of the amount of dye diffused from the samples.
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