User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/08/27: Difference between revisions
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==August 27, 2014== | |||
==Preparation of Stock Solutions & Culture Tubes== | ==Preparation of Stock Solutions & Culture Tubes== | ||
We made 50 mL of a 2.512 mM solution of HAuCl<sub>4</sub>. | We made 50 mL of a 2.512 mM solution of HAuCl<sub>4</sub>. | ||
Line 22: | Line 23: | ||
==<font color=#0f1028></font>== | ==<font color=#0f1028></font>== | ||
{|style="width:700px" | {|style="width:700px" | ||
|<u>Au (mL)</u> | |<u>'''Au (mL)'''</u> | ||
|<u>Lysozyme (mL)</u> | |<u>'''Lysozyme (mL)'''</u> | ||
|<u>H<sub>2</sub>O (mL)</u> | |<u>'''H<sub>2</sub>O (mL)'''</u> | ||
|<u>[Au]:[lysozyme]</u> | |<u>'''[Au]:[lysozyme]'''</u> | ||
|- | |- | ||
|2.5 | |2.5 |
Latest revision as of 00:12, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||
August 27, 2014Preparation of Stock Solutions & Culture TubesWe made 50 mL of a 2.512 mM solution of HAuCl4.
We then made 50 mL of a 250 μM solution of lysozyme.
Finally, we made 50 mL of a 2.512 x 10-4 M solution of HCl.
NOTE: this needs to be re-done to match the concentration of the HAuCl4 stock, or 2.512 mM. 6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.
NOTE: The amount of Au in each tube was intended to be 0.5 mL, or 1/10 the volume of the final solution. Using a stock solution with a [H+] of 2.512 mM, the concentration of [H+] in the final 5 mL solution would be 2.512 x 10-4. This would give a solution of 3.6. Because we used 2.5 mL of Au in each tube, or 1/2 the volume of the final solution, our culture tubes contained a higher [H+] and therefore had a lower pH of 2.9. Each test tube was covered in foil and placed in an oven at 80°C for 3 hours. Kinetics of AuNP Formation
|