User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==September 24, 2014== | ||
==Bradford Analysis of Dialysis== | |||
A Bradford analysis was done on the dialysis samples prepared and dialyzed last week. | |||
*600 µL of each soak solution were combined with 200 µL of Bradford reagent and 200 µL of a 50 mM Tris/NaCl buffer | |||
**The soak solutions included: | |||
50 mM NaCl stock solution with 3500 g/mol MWCO tubing | |||
50 mM Glycine stock solution with 3500 g/mol MWCO tubing | |||
Pure water with 3500 g/mol MWCO tubing | |||
50 mM Glycine stock solution with 25k g/mol MWCO tubing | |||
50 mM Glycine stock solution with 3500 g/mol MWCO tubing | |||
*100 µL of the contents of each dialysis tubing was combined with 200 µL of Bradford reagent and 700 µL of Tris/NaCl buffer | |||
**The respective dialysis bag contents were analyzed, all of which initially included 4 mL of 1g/L lysozyme stock solution, except for the very last system, in which 4.0 mL of 36.5 [Au]:[Lys] colloid solution was used in the place of lysozyme against the 50 mM glycine soak with 3500 tubing | |||
'''NOTE''': As mentioned previously, the contents of the tube in the fourth system (50 mM glycine solution with 25K g/mol MWCO tubing) were lost during transfer; thus, only four tubing samples are included in the results. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Solution''' | |||
| align="center" style="background:#f0f0f0;"|'''Conc of Lysozyme (µg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Abs''' | |||
|- | |||
| 3500 NaCl Soak||-3.344660194||0.028 | |||
|- | |||
| 3500 H2O Soak||-3.975728155||0.015 | |||
|- | |||
| 3500 Gly Soak||2.529126214||0.149 | |||
|- | |||
| 25k Gly Soak||-0.09223301||0.095 | |||
|- | |||
| 3500 Gly Colloid||1.703883495||0.132 | |||
|- | |||
| 3500 NaCl/Lys Bag||17.91747573||0.466 | |||
|- | |||
| 3500 H2O/ Lys Bag||18.54854369||0.479 | |||
|- | |||
| 3500 Gly/Lys Bag||20.05339806||0.51 | |||
|- | |||
| 3500 Gly/Coll Bag||2.82038835||0.155 | |||
|} | |||
[[Image:Bradford_Dialysis_Analysis.png|500px]] | |||
'''NOTE''': Negative concentrations of protein in the soak solutions may be a result of protein precipitation. | |||
==CaCl<sub>2</sub> Conductivity== | |||
A hard water probe sensitive only to specific polyvalent cations such as Ca<sup>2+</sup> was used to measure the conductivity in mV of 5 solutions of CaCl<sub>2</sub> of various concentrations. | |||
*0.11098 g of CaCl<sub>2</sub> was dissolved in 20 mL of water, making a 50 mM CaCl<sub>2</sub> solution. 2 mL of this solution was then diluted with 18 mL of water to make the next serial dilution. This step was repeated 3 more times. | |||
==<font color=#0f1028></font>== | |||
{|style="width:700px" | |||
|<u>'''Concentration'''</u> | |||
|<u>'''Conductivity (mV)'''</u> | |||
|- | |||
|5 µM | |||
|(-4.1) | |||
|- | |||
|50 µM | |||
|7.3 | |||
|- | |||
|0.5 mM | |||
|36.1 | |||
|- | |||
|5 mM | |||
|63.6 | |||
|- | |||
|50 mM | |||
|87.8 | |||
|- | |||
|} | |||
'''NOTE''': The results were graphed with conductivity as a function of the log of CaCl<sub>2</sub> concentration. | |||
[[Image:CaCl2_Conductivity.png|500px]] | |||
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Latest revision as of 00:21, 27 September 2017
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September 24, 2014Bradford Analysis of DialysisA Bradford analysis was done on the dialysis samples prepared and dialyzed last week.
50 mM NaCl stock solution with 3500 g/mol MWCO tubing 50 mM Glycine stock solution with 3500 g/mol MWCO tubing Pure water with 3500 g/mol MWCO tubing 50 mM Glycine stock solution with 25k g/mol MWCO tubing 50 mM Glycine stock solution with 3500 g/mol MWCO tubing
NOTE: As mentioned previously, the contents of the tube in the fourth system (50 mM glycine solution with 25K g/mol MWCO tubing) were lost during transfer; thus, only four tubing samples are included in the results.
NOTE: Negative concentrations of protein in the soak solutions may be a result of protein precipitation. CaCl2 ConductivityA hard water probe sensitive only to specific polyvalent cations such as Ca2+ was used to measure the conductivity in mV of 5 solutions of CaCl2 of various concentrations.
NOTE: The results were graphed with conductivity as a function of the log of CaCl2 concentration. |