User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| 3500 Gly/Coll Bag||2.82038835||0.155 | | 3500 Gly/Coll Bag||2.82038835||0.155 | ||
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[[Image:Bradford_Dialysis_Analysis.png|500px]] | |||
'''NOTE''': Negative concentrations of protein in the soak solutions may be a result of protein precipitation. | |||
==CaCl<sub>2</sub> Conductivity== | |||
A hard water probe sensitive only to specific polyvalent cations such as Ca<sup>2+</sup> was used to measure the conductivity in mV of 5 solutions of CaCl<sub>2</sub> of various concentrations. | |||
*0.11098 g of CaCl<sub>2</sub> was dissolved in 20 mL of water, making a 50 mM CaCl<sub>2</sub> solution. 2 mL of this solution was then diluted with 18 mL of water to make the next serial dilution. This step was repeated 3 more times. | |||
==<font color=#0f1028></font>== | |||
{|style="width:700px" | |||
|<u>'''Concentration'''</u> | |||
|<u>'''Conductivity (mV)'''</u> | |||
|- | |||
|5 µM | |||
|(-4.1) | |||
|- | |||
|50 µM | |||
|7.3 | |||
|- | |||
|0.5 mM | |||
|36.1 | |||
|- | |||
|5 mM | |||
|63.6 | |||
|- | |||
|50 mM | |||
|87.8 | |||
|- | |||
|} | |} | ||
'''NOTE''': The results were graphed with conductivity as a function of the log of CaCl<sub>2</sub> concentration. | |||
[[Image:CaCl2_Conductivity.png|500px]] | |||
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Latest revision as of 00:21, 27 September 2017
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September 24, 2014Bradford Analysis of DialysisA Bradford analysis was done on the dialysis samples prepared and dialyzed last week.
50 mM NaCl stock solution with 3500 g/mol MWCO tubing 50 mM Glycine stock solution with 3500 g/mol MWCO tubing Pure water with 3500 g/mol MWCO tubing 50 mM Glycine stock solution with 25k g/mol MWCO tubing 50 mM Glycine stock solution with 3500 g/mol MWCO tubing
NOTE: As mentioned previously, the contents of the tube in the fourth system (50 mM glycine solution with 25K g/mol MWCO tubing) were lost during transfer; thus, only four tubing samples are included in the results.
NOTE: Negative concentrations of protein in the soak solutions may be a result of protein precipitation. CaCl2 ConductivityA hard water probe sensitive only to specific polyvalent cations such as Ca2+ was used to measure the conductivity in mV of 5 solutions of CaCl2 of various concentrations.
NOTE: The results were graphed with conductivity as a function of the log of CaCl2 concentration. |