User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/24: Difference between revisions
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== | ==September 24, 2014== | ||
* | |||
==Bradford Analysis of Dialysis== | |||
A Bradford analysis was done on the dialysis samples prepared and dialyzed last week. | |||
*600 µL of each soak solution were combined with 200 µL of Bradford reagent and 200 µL of a 50 mM Tris/NaCl buffer | |||
**The soak solutions included: | |||
50 mM NaCl stock solution with 3500 g/mol MWCO tubing | |||
50 mM Glycine stock solution with 3500 g/mol MWCO tubing | |||
Pure water with 3500 g/mol MWCO tubing | |||
50 mM Glycine stock solution with 25k g/mol MWCO tubing | |||
50 mM Glycine stock solution with 3500 g/mol MWCO tubing | |||
*100 µL of the contents of each dialysis tubing was combined with 200 µL of Bradford reagent and 700 µL of Tris/NaCl buffer | |||
**The respective dialysis bag contents were analyzed, all of which initially included 4 mL of 1g/L lysozyme stock solution, except for the very last system, in which 4.0 mL of 36.5 [Au]:[Lys] colloid solution was used in the place of lysozyme against the 50 mM glycine soak with 3500 tubing | |||
'''NOTE''': As mentioned previously, the contents of the tube in the fourth system (50 mM glycine solution with 25K g/mol MWCO tubing) were lost during transfer; thus, only four tubing samples are included in the results. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Solution''' | |||
| align="center" style="background:#f0f0f0;"|'''Conc of Lysozyme (µg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Abs''' | |||
|- | |||
| 3500 NaCl Soak||-3.344660194||0.028 | |||
|- | |||
| 3500 H2O Soak||-3.975728155||0.015 | |||
|- | |||
| 3500 Gly Soak||2.529126214||0.149 | |||
|- | |||
| 25k Gly Soak||-0.09223301||0.095 | |||
|- | |||
| 3500 Gly Colloid||1.703883495||0.132 | |||
|- | |||
| 3500 NaCl/Lys Bag||17.91747573||0.466 | |||
|- | |||
| 3500 H2O/ Lys Bag||18.54854369||0.479 | |||
|- | |||
| 3500 Gly/Lys Bag||20.05339806||0.51 | |||
|- | |||
| 3500 Gly/Coll Bag||2.82038835||0.155 | |||
|} | |||
Revision as of 10:34, 28 September 2014
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September 24, 2014Bradford Analysis of DialysisA Bradford analysis was done on the dialysis samples prepared and dialyzed last week.
50 mM NaCl stock solution with 3500 g/mol MWCO tubing 50 mM Glycine stock solution with 3500 g/mol MWCO tubing Pure water with 3500 g/mol MWCO tubing 50 mM Glycine stock solution with 25k g/mol MWCO tubing 50 mM Glycine stock solution with 3500 g/mol MWCO tubing
NOTE: As mentioned previously, the contents of the tube in the fourth system (50 mM glycine solution with 25K g/mol MWCO tubing) were lost during transfer; thus, only four tubing samples are included in the results.
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