User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/27: Difference between revisions
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== | ==January 27th, 2015== | ||
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=='''Stock Solution Preparation'''== | |||
1. 100 mL of '''2.5 mM HAuCl<sub>4</sub>''' solution was made from 0.08539 g of HAuCl4 (MW 339.785 g/mol) in 100 mL of distilled H<sub>2</sub>O | |||
2. 100 mL of '''20 μM lysozyme''' solution was made from 0.02861 g of lysozyme (MW 14300 Da) in 100 mL of distilled H<sub>2</sub>O | |||
==AuNP Fiber Preparation== | |||
50 - 5 mL tubes of 45:1 [Au]:[Lysozme] solution were prepared with final concentrations of 0.25 mM and 5.56 μM of Au and lysozyme, respectively. | |||
Each tubed contained the following: | |||
*0.5 mL of 2.5 mM HAuCl<sub>4</sub> | |||
*1.39 mL of 20 μM lysozyme | |||
*3.11 mL of distilled H<sub>2</sub>O | |||
The AuNP solutions were placed in the oven at 80°C for four hours. | |||
=Protease and Buffer Preparation== | |||
1. 8 samples of approximately 4 mg of pepsin were prepared. | |||
*1. 0.00530 g | |||
*2. 0.00398 g | |||
*3. 0.00391 g | |||
*4. 0.00470 g | |||
*5. 0.00419 g | |||
*6. 0.00465 g | |||
*7. 0.00780 g | |||
*8. 0.00589 g | |||
2. 1000 mL of 5 mM Tris/0.2 M NaCl buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H<sub>2</sub>. 0.5 N HCl was added dropwise to a pH of 8.067. | |||
Revision as of 18:19, 27 January 2015
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January 27th, 2015Stock Solution Preparation1. 100 mL of 2.5 mM HAuCl4 solution was made from 0.08539 g of HAuCl4 (MW 339.785 g/mol) in 100 mL of distilled H2O 2. 100 mL of 20 μM lysozyme solution was made from 0.02861 g of lysozyme (MW 14300 Da) in 100 mL of distilled H2O AuNP Fiber Preparation50 - 5 mL tubes of 45:1 [Au]:[Lysozme] solution were prepared with final concentrations of 0.25 mM and 5.56 μM of Au and lysozyme, respectively. Each tubed contained the following:
The AuNP solutions were placed in the oven at 80°C for four hours. Protease and Buffer Preparation=1. 8 samples of approximately 4 mg of pepsin were prepared.
2. 1000 mL of 5 mM Tris/0.2 M NaCl buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H2. 0.5 N HCl was added dropwise to a pH of 8.067.
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