User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/27: Difference between revisions
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The AuNP solutions were placed in the oven at 80°C for four hours. | The AuNP solutions were placed in the oven at 80°C for four hours. | ||
=Protease and Buffer Preparation== | =='''Protease and Buffer Preparation'''== | ||
1. 8 samples of approximately 4 mg of pepsin were prepared. | 1. 8 samples of approximately '''4 mg of pepsin''' were prepared. | ||
*1. 0.00530 g | *1. 0.00530 g | ||
*2. 0.00398 g | *2. 0.00398 g | ||
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*8. 0.00589 g | *8. 0.00589 g | ||
2. 1000 mL of 5 mM Tris/0.2 M NaCl buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H<sub>2</sub>. 0.5 N HCl was added dropwise to a pH of 8.067. | 2. 1000 mL of '''5 mM Tris/0.2 M NaCl''' buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H<sub>2</sub>. 0.5 N HCl was added dropwise to a '''pH of 8.067'''. | ||
Revision as of 18:21, 27 January 2015
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January 27th, 2015Stock Solution Preparation1. 100 mL of 2.5 mM HAuCl4 solution was made from 0.08539 g of HAuCl4 (MW 339.785 g/mol) in 100 mL of distilled H2O 2. 100 mL of 20 μM lysozyme solution was made from 0.02861 g of lysozyme (MW 14300 Da) in 100 mL of distilled H2O AuNP Fiber Preparation50 - 5 mL tubes of 45:1 [Au]:[Lysozme] AuNP solution were prepared with final concentrations of 0.25 mM and 5.56 μM of Au and lysozyme, respectively. Each tubed contained the following:
The AuNP solutions were placed in the oven at 80°C for four hours. Protease and Buffer Preparation1. 8 samples of approximately 4 mg of pepsin were prepared.
2. 1000 mL of 5 mM Tris/0.2 M NaCl buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H2. 0.5 N HCl was added dropwise to a pH of 8.067.
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