User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/28
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January 28th, 2015Pepsin Reaction PreparationThe 50 - 5 mL samples of AuNPs were observed for the formation of fiber aggregates. The presence of these fibers was confirmed. 8 of these 5 mL samples were taken and the supernatant solution was drained using a disposable pipet. Not all 5 mL of solution was removed from each test tube --> While majority of the fibers stuck to the walls of the glass tubes, some were free in solution and were susceptible to being sucked up with the supernatant. NOTE: Concentrations of pepsin were thus altered from the values intended, which are demonstrated below. After removing the supernatant, a proper amount of pepsin in 5 mM Tris*HCl/0.2 M NaCl (pH 8) buffer was added to each of the 8 samples with the volumes and concentrations specified below:
Reaction time: 4 hours 2. 0.00398 g of pepsin
Reaction time: 6 days
Reaction time: 4 hours 4. 0.00470 g of pepsin
Reaction time: 6 days
Reaction time: 4 hours 6. 0.00465 g of pepsin
Reaction time: 6 days
Reaction time: 4 hours 8. 0.00584 g of pepsin
Reaction time: 6 days
Approximately 2 hours into the reaction, no visible color change was observed. |