User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/03/03
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Entry titlePepsin Reaction Preparation0.00166 g of pepsin was dissolved in 1 mL of 5 mM citrate/0.2 M NaCl buffer ---> stock [pepsin] = 47.98 μM Bradford/Gel Analysis samples to be run: 1. 0.104(2) mL of stock solution in 4.896 mL of citrate/NaCl buffer
2. 0.010(4) mL of stock solution in 4.989 mL of citrate/NaCl buffer
3. 0.001(0) mL of stock solution in 4.999 mL of citrate/NaCl buffer
4. 10 μL of stock solution in 990 μL of Tris/NaCl buffer --> [trypsin] = 0.4798 μM
Thermolysin Reaction Preparation0.00063 g of Chymotrypsin was dissolved in 1 mL of Tris/NaCl buffer ---> stock [thermolysin] = 18.21 μM Bradford/Gel Analysis samples to be run: 1. 0.274(6) mL of stock solution in 4.725 mL of Tris/NaCl buffer
2. 0.027(4) mL of stock solution in 4.973 mL of Tris/NaCl buffer
3. 0.002(7) mL of stock solution in 4.997 mL of Tris/NaCl buffer
4. 10 μL of stock solution in 990 μL of Tris/NaCl buffer --> [trypsin] = 0.1821 μM
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