Checking Sequencing Data
GFP vs RFP
- E06 and E07 a little funky, not lining up well with RFP. E06 has almost no matching, E07 only a little strange, not very far off.
- R3.2 should be RFP, are in fact in a GFP backbone. However, R3.1 is on RFP, so it should be fine.
- R7.1 sequenced weird, R 7.2 has some mutations
- R6.1 no match to RFP, but R6.2 is ok
- R10.1 is gibberish, should be RFP. However, R10.2 is RFP, so it should be fine.
- R22.1, R22.2, R23.1, R23.2, R24.1 and R24.2 are in GFP backbones
- B04 (G4.2) does not map onto GFP at all. However, G4.1 does, so I shouldn't have a problem here.
- B04 (G4.2) matches well to RFP.
- G4.2 is in RFP, but it should be ok, because G4.1 is ok (ie, in GFP).
- Otherwise, all G's are ok
R2.2 ok
R1.2 ok
- R6.1 sequenced weirdly. However, R6.2 lines up well with RFP, so it should be fine.
Questions on:
- R10.1
- R10.2 (SNP ok?)
- R6.1
Need to re-do in some way:
- R7 (7.1 sequenced weird, 7.2 has some substitution mutations)
- R16 (no colonies)
- R17 (no colonies)
- R18 (no colonies)
- R22 (both GFP)
- R23 (both GFP)
- R24 (both GFP)
- might be worth re-doing R20, R10
T7 promoter
- present in all R's
- present in all G's
Makoff RBS part 1
- present in all G's
- present in all R's
Good sequences
- All G clones are good, except for G4.2
- R1.1 (R1.2 ok - SNP)
- R2.1 (R2.2 ok - SNP)
- R3.1
- R4.1 or R4.2
- R5.1 or R5.2
- R6.2 (R6.1 gibberish)
- R8.1 or R8.2
- R9.1 or R9.2
- R10.2 (ok - has SNP)
- R11.1 or R11.2
- R12.1 or R12.2
- R13.1 (R13.2 ok - SNP)
- R14.1 (R14.2 ok - SNP)
- R15.1 (R15.2 ok - SNP)
- R19.1 or R19.2
- R20.1 or R20.2 (minor SNPs in both)
- R21.1 or R21.2
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C.Dog T7 Promoter Project
