User:Morgan L. Paull/Notebook/C.Dog V1.0 - T7 Promoter/2011/07/12: Difference between revisions
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==Checking the sequences for re-picked colonies from the last round of ligations== | ==Checking the sequences for re-picked colonies from the last round of ligations== | ||
* All G12's have GFP and T7, but do not have either Makoff RBS part 1 or part 12. | * All G12's have GFP and T7, but do not have either Makoff RBS part 1 or part 12. | ||
--> G12 first ligation is no good. | |||
* All R10's have RFP. Some SNPs in sequencing, but all explainable. Nonetheless, R10.4 appears to be the safest bet from the sequencing results (only SNPs are completely explainable by base repeats). R10.3 is a little funky on the alignment to makoff 10, but probably ok. R10.4 and R10.5 definitely ok. T7 and makoff BD part 1 both present in all three. | * All R10's have RFP. Some SNPs in sequencing, but all explainable. Nonetheless, R10.4 appears to be the safest bet from the sequencing results (only SNPs are completely explainable by base repeats). R10.3 is a little funky on the alignment to makoff 10, but probably ok. R10.4 and R10.5 definitely ok. T7 and makoff BD part 1 both present in all three. | ||
--> R10.4 and R10.5 both good to go. | |||
* | * | ||
Revision as of 12:52, 12 July 2011
 C.Dog T7 Promoter Project
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Checking the sequences for re-picked colonies from the last round of ligations
--> G12 first ligation is no good.
--> R10.4 and R10.5 both good to go.
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