User:Mradha: Difference between revisions
No edit summary |
No edit summary |
||
| Line 77: | Line 77: | ||
Refactoring M13 | Refactoring M13 | ||
| | |||
I created three new parts on the Registry of Standard Biological Parts BBa_M31000, M31001, and M31002. M31000 contains the entire DNA sequence from the Hpal site to the BamH1 site. I removed the Hpal site and changed it into another BamH1 site. This allows for this portion of the plasmid to cut up and removed from the rest of the genome. It will also have blunt ends but the ends will anneal to the each other. I removed gene 7 and left gene 8's promoter and gene 9's RBS. In M31001 I added gene X's promoter and RBS to the front of gene X and made it a compact and independent gene. Then in M31002 I changed gene 2 so that gene 10's promoter and RBS were disrupted and unfunctional. This made it so that gene 2 can be manipulated individually compared to gene 10. | I created three new parts on the Registry of Standard Biological Parts BBa_M31000, M31001, and M31002. M31000 contains the entire DNA sequence from the Hpal site to the BamH1 site. I removed the Hpal site and changed it into another BamH1 site. This allows for this portion of the plasmid to cut up and removed from the rest of the genome. It will also have blunt ends but the ends will anneal to the each other. I removed gene 7 and left gene 8's promoter and gene 9's RBS. In M31001 I added gene X's promoter and RBS to the front of gene X and made it a compact and independent gene. Then in M31002 I changed gene 2 so that gene 10's promoter and RBS were disrupted and unfunctional. This made it so that gene 2 can be manipulated individually compared to gene 10. | ||
Revision as of 23:40, 26 February 2007
Protein Modifications
| protein | function |
|---|---|
| I | Modify protein so that similar proteins, other than pVIII can bind to the surface. |
| II | Keep track of the number of proteins that it nicks. |
| III | Make the proteins bigger so that they can bind to objects easier and so that they can also bind to bigger objects. |
| IV | Modify protein so that similar proteins, other than pVIII can bind to the surface. |
| V | Put tag on the protein so that you can track the bacteria stages. |
| VI | Make it bind more tightly to p3, so that p3 can be modified easily and so that p3 is much more flexible. |
| VII | Make is bind more tightly to p9 to allow for more flexibility. |
| VIII | Make fewer copies so that the proteins can be more flexible. |
| IX | Change the function so that it now lyse the bacteria. |
| X | Increase the number of proteins so that the phage can produce more double strands. |
| XI | Modify protein so that similar proteins, other than pVIII can bind to the surface. |
Results of Ligation Reactions
| protein | function |
|---|---|
| Digest 1: Candidate 1 | One band visible. |
| Digest 1: Candidate 2 | One band visible. |
| Digest 1: Candidate 3 | Two visible bands. |
| Digest 1: Candidate 4 | Two visible bands. |
| Digest 2: Candidate 1 | Four visible bands. |
| Digest 2: Candidate 2 | Three visible bands. |
| Digest 2: Candidate 3 | Three vibible bands. |
| Digest 2: Candidate 4 | Two bands visible but one might just be noise. |
Refactoring M13
|
I created three new parts on the Registry of Standard Biological Parts BBa_M31000, M31001, and M31002. M31000 contains the entire DNA sequence from the Hpal site to the BamH1 site. I removed the Hpal site and changed it into another BamH1 site. This allows for this portion of the plasmid to cut up and removed from the rest of the genome. It will also have blunt ends but the ends will anneal to the each other. I removed gene 7 and left gene 8's promoter and gene 9's RBS. In M31001 I added gene X's promoter and RBS to the front of gene X and made it a compact and independent gene. Then in M31002 I changed gene 2 so that gene 10's promoter and RBS were disrupted and unfunctional. This made it so that gene 2 can be manipulated individually compared to gene 10.
