User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/09/18: Difference between revisions
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==Procedure== | ==Procedure== | ||
#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on | #10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on | ||
#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve | #After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling | ||
#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for | #After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place | ||
#Then the flask was cooled in ice for 15min | #Then the flask was cooled in ice bath for 15min | ||
#20ml of concentrated HCl was added to the solution | #20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min | ||
#The solution was filtered | #The solution was filtered | ||
Revision as of 11:32, 27 September 2012
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Objective
Zinc acetate was added to protoporphyrin ix to place the metal in the middle of the protoporphyrin ix structure
Procedure
- 10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on
- After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling
- After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place
- Then the flask was cooled in ice bath for 15min
- 20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min
- The solution was filtered
