User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/09/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Procedure== | ==Procedure== | ||
#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on | #10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on a heating mantle and under a condenser. | ||
#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve | #After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling. | ||
#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for | #After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place. | ||
#Then the flask was cooled in ice for 15min | #Then the flask was cooled in ice bath for 15min. | ||
#20ml of concentrated HCl was added to the solution | #20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min. | ||
#The solution was filtered | #The solution was filtered. | ||
#No precipitate was formed on the filter paper. | |||
#The solution was gathered again and was allowed to run under nitrogen gas pressure for 24 hours. | |||
#The solution was filtered again and again no precipitate was formed on the filter paper. | |||
Latest revision as of 22:04, 26 September 2017
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Objective
Zinc acetate was added to protoporphyrin ix to place the metal in the middle of the protoporphyrin ix structure
Procedure
- 10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on a heating mantle and under a condenser.
- After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling.
- After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place.
- Then the flask was cooled in ice bath for 15min.
- 20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min.
- The solution was filtered.
- No precipitate was formed on the filter paper.
- The solution was gathered again and was allowed to run under nitrogen gas pressure for 24 hours.
- The solution was filtered again and again no precipitate was formed on the filter paper.
