User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/09/18 - Revision history

NYOUSHA Yousefi: /* Procedure */

2012-09-27T18:49:56Z

Procedure

←Older revision		Revision as of 18:49, 27 September 2012
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	==Procedure==		==Procedure==
-	#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on	+	#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on a heating mantle and under a condenser.
-	#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling	+	#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling.
-	#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place	+	#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place.
-	#Then the flask was cooled in ice bath for 15min	+	#Then the flask was cooled in ice bath for 15min.
-	#20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min	+	#20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min.
-	#The solution was filtered	+	#The solution was filtered.
		+	#No precipitate was formed on the filter paper.
		+	#The solution was gathered again and was allowed to run under nitrogen gas pressure for 24 hours.
		+	#The solution was filtered again and again no precipitate was formed on the filter paper.

NYOUSHA Yousefi: /* Procedure */

2012-09-27T18:32:54Z

Procedure

←Older revision		Revision as of 18:32, 27 September 2012
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	==Procedure==		==Procedure==
	#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on		#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on
-	#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve ~~for~~ for 20min while still boiling	+	#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling
-	#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for ~~30 more min~~ to allow the reaction take place	+	#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place
-	#Then the flask was cooled in ice for 15min	+	#Then the flask was cooled in ice bath for 15min
-	#20ml of concentrated HCl was added to the solution	+	#20ml of concentrated HCl was added to the solution and was placed in the ice bath for another 10min
	#The solution was filtered		#The solution was filtered

NYOUSHA Yousefi: /* Procedure */

2012-09-27T18:31:18Z

Procedure

←Older revision		Revision as of 18:31, 27 September 2012
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	==Procedure==		==Procedure==
		+	#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on
		+	#After it started boiling, 0.1059g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for for 20min while still boiling
		+	#After 20min, 0.0226g of zinc acetate was added to the solution and it was heated for 30 more min to allow the reaction take place
		+	#Then the flask was cooled in ice for 15min
		+	#20ml of concentrated HCl was added to the solution
		+	#The solution was filtered

NYOUSHA Yousefi: /* Objective */

2012-09-27T18:22:48Z

Objective

←Older revision		Revision as of 18:22, 27 September 2012
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	==Objective==		==Objective==
		+	Zinc acetate was added to protoporphyrin ix to place the metal in the middle of the protoporphyrin ix structure
		+
		+
		+	==Procedure==

NYOUSHA Yousefi: /* Entry title */

2012-09-27T18:21:35Z

Entry title

←Older revision		Revision as of 18:21, 27 September 2012
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	==Entry title==		==Entry title==
-	* Protoporphyrin IX and Zinc acetate day 1	+	* Protoporphyrin IX and Zinc acetate, day 1
		+

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	__NOTOC__		__NOTOC__
		+
	==Objective==		==Objective==

NYOUSHA Yousefi: /* Entry title */

2012-09-27T18:21:15Z

Entry title

←Older revision		Revision as of 18:21, 27 September 2012
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	==Entry title==		==Entry title==
-	* Protoporphyrin IX and Zinc acetate	+	* Protoporphyrin IX and Zinc acetate day 1

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	__NOTOC__		__NOTOC__
		+	==Objective==

NYOUSHA Yousefi: /* Entry title */

2012-09-27T18:19:40Z

Entry title

←Older revision		Revision as of 18:19, 27 September 2012
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	==Entry title==		==Entry title==
	* Protoporphyrin IX and Zinc acetate		* Protoporphyrin IX and Zinc acetate
-	~~==Objective==~~

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

NYOUSHA Yousefi: /* Entry title */

2012-09-27T18:19:27Z

Entry title

←Older revision		Revision as of 18:19, 27 September 2012
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	==Entry title==		==Entry title==
	* Protoporphyrin IX and Zinc acetate		* Protoporphyrin IX and Zinc acetate
-		+	==Objective==

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

NYOUSHA Yousefi: /* Entry title */

2012-09-27T18:17:52Z

Entry title

←Older revision		Revision as of 18:17, 27 September 2012
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	==Entry title==		==Entry title==
-	* ~~Insert content here...~~	+	* Protoporphyrin IX and Zinc acetate

NYOUSHA Yousefi: Autocreate 2012/09/18 Entry for User:NYOUSHA_Yousefi/Notebook/Protein_Engineering

2012-09-27T18:16:19Z

Autocreate 2012/09/18 Entry for User:NYOUSHA_Yousefi/Notebook/Protein_Engineering

New page

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==Entry title==
* Insert content here...


|}

__NOTOC__