User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/09/25: Difference between revisions
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The Procedure performed on day 1 was repeated with slight changes | The Procedure performed on day 1 was repeated with slight changes | ||
==Procedure== | ==Procedure== | ||
#10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on | |||
#After it started boiling, 0.1006g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling | |||
#After 20min, 0.0230g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place | |||
#Then the flask was cooled in ice bath for 15min | |||
#100ml of cooled distilled water was added to the solution | |||
#A Filter paper was measured before filtration, it weighed 0.0706g | |||
#The solution was filtered with a different filter paper with much smaller filter pores | |||
#The precipitate and the filter paper were place on a plate and were raped by aluminum paper to keep it away from light | |||
#The filter paper and the precipitant were allowed to completely dry out to be ready for final mass measurements | |||
Revision as of 11:43, 27 September 2012
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Entry titleProtoporphyrin IX and Zinc acetate, day 2
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Objective
The Procedure performed on day 1 was repeated with slight changes
Procedure
- 10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on
- After it started boiling, 0.1006g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling
- After 20min, 0.0230g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place
- Then the flask was cooled in ice bath for 15min
- 100ml of cooled distilled water was added to the solution
- A Filter paper was measured before filtration, it weighed 0.0706g
- The solution was filtered with a different filter paper with much smaller filter pores
- The precipitate and the filter paper were place on a plate and were raped by aluminum paper to keep it away from light
- The filter paper and the precipitant were allowed to completely dry out to be ready for final mass measurements
