User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/10/09: Difference between revisions
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==Objective== | ==Objective== | ||
The frozen cells that were presented with Zn Hb were prepared for further protein expression studies | The frozen cells, that were presented with Zn Hb and were allowed to replicate, were prepared for further protein expression studies | ||
==Procedure== | ==Procedure== | ||
#The zinc protoporphyrin prepared in previous labs was presented to cultured cells. | |||
#The containers of the frozen cells were taken out of the freezer and were allowed to slowly cool down at the room temperature in a beaker full of tap water. | |||
#When the cells mixture completely turned into liquid, the cells were killed and broken apart by a ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times. | |||
#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to three decimal places. | |||
#The cells were centrifuges at 18000g for two hours. | |||
#After the centrifuge, the supernatant was separated and stored in different tubes. | |||
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Revision as of 07:17, 16 October 2012
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Entry titleProtein expression ObjectiveThe frozen cells, that were presented with Zn Hb and were allowed to replicate, were prepared for further protein expression studies Procedure
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