User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/10/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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==Procedure== | ==Procedure== | ||
#The containers frozen cells were taken out of the freezer and were allowed to slowly cool down at the room temperature in a beaker full of tap water. | #The zinc protoporphyrin prepared in previous labs was presented to cultured cells. | ||
#When the cells mixture completely turned into liquid, | #The containers of the frozen cells were taken out of the freezer and were allowed to slowly cool down at the room temperature in a beaker full of tap water. | ||
#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to | #When the cells mixture completely turned into liquid, the cells were killed and broken apart by a ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times. | ||
#The cells were centrifuges at 18000g for two hours. | #The cell mixtures were placed into special centrifuge tubes and their weights were balanced to three decimal places. | ||
#The cells were centrifuges at 18000g for two hours. | |||
#After the centrifuge, the supernatant was separated and stored in different tubes. | |||
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Latest revision as of 22:06, 26 September 2017
Project name | Main project page Previous entry Next entry |
Entry titleProtein expression ObjectiveThe frozen cells, that were presented with Zn Hb and were allowed to replicate, were prepared for further protein expression studies Procedure
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