User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/10/09 - Revision history

NYOUSHA Yousefi: /* Procedure */

NYOUSHA Yousefi — Tue, 16 Oct 2012 14:17:42 GMT

Procedure

←Older revision		Revision as of 14:17, 16 October 2012
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	==Procedure==		==Procedure==
-	#The containers frozen cells were taken out of the freezer and were allowed to slowly cool down at the room temperature in a beaker full of tap water.	+	#The zinc protoporphyrin prepared in previous labs was presented to cultured cells.
-	#When the cells mixture completely turned into liquid, ~~they~~ were killed and broken apart by ~~the~~ ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times.	+	#The containers of the frozen cells were taken out of the freezer and were allowed to slowly cool down at the room temperature in a beaker full of tap water.
-	#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to ~~hundred~~ decimal places.	+	#When the cells mixture completely turned into liquid, the cells were killed and broken apart by a ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times.
		+	#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to three decimal places.
	#The cells were centrifuges at 18000g for two hours.		#The cells were centrifuges at 18000g for two hours.
	#After the centrifuge, the supernatant was separated and stored in different tubes.		#After the centrifuge, the supernatant was separated and stored in different tubes.

NYOUSHA Yousefi: /* Procedure */

NYOUSHA Yousefi — Tue, 09 Oct 2012 20:47:05 GMT

Procedure

←Older revision		Revision as of 20:47, 9 October 2012
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	#When the cells mixture completely turned into liquid, they were killed and broken apart by the ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times.		#When the cells mixture completely turned into liquid, they were killed and broken apart by the ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times.
	#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to hundred decimal places.		#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to hundred decimal places.
-	#The cells were centrifuges at 18000g for two hours.	+	#The cells were centrifuges at 18000g for two hours.
		+	#After the centrifuge, the supernatant was separated and stored in different tubes.
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NYOUSHA Yousefi: /* Objective */

NYOUSHA Yousefi — Tue, 09 Oct 2012 19:35:16 GMT

Objective

←Older revision		Revision as of 19:35, 9 October 2012
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	==Objective==		==Objective==
-	The frozen cells that were presented with Zn Hb were prepared for further protein expression studies	+	The frozen cells, that were presented with Zn Hb and were allowed to replicate, were prepared for further protein expression studies

	==Procedure==		==Procedure==

NYOUSHA Yousefi: /* Procedure */

NYOUSHA Yousefi — Tue, 09 Oct 2012 19:33:57 GMT

Procedure

←Older revision		Revision as of 19:33, 9 October 2012
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	==Procedure==		==Procedure==
-		+	#The containers frozen cells were taken out of the freezer and were allowed to slowly cool down at the room temperature in a beaker full of tap water.
		+	#When the cells mixture completely turned into liquid, they were killed and broken apart by the ultrasound processor in 30seconds intervals of ultrasound and 30s intervals of sitting still in the ice. This intervals were repeated for 3 times.
		+	#The cell mixtures were placed into special centrifuge tubes and their weights were balanced to hundred decimal places.
		+	#The cells were centrifuges at 18000g for two hours.
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

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NYOUSHA Yousefi: /* Objective */

NYOUSHA Yousefi — Tue, 09 Oct 2012 19:11:46 GMT

Objective

←Older revision		Revision as of 19:11, 9 October 2012
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	==Objective==		==Objective==
		+	The frozen cells that were presented with Zn Hb were prepared for further protein expression studies

		+	==Procedure==

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NYOUSHA Yousefi: /* Entry title */

NYOUSHA Yousefi — Tue, 09 Oct 2012 19:09:42 GMT

Entry title

←Older revision		Revision as of 19:09, 9 October 2012
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	==Entry title==		==Entry title==
-	* Insert content here...	+	Protein expression
		+
		+	==Objective==

NYOUSHA Yousefi: Autocreate 2012/10/09 Entry for User:NYOUSHA_Yousefi/Notebook/Protein_Engineering

NYOUSHA Yousefi — Tue, 09 Oct 2012 19:08:31 GMT

Autocreate 2012/10/09 Entry for User:NYOUSHA_Yousefi/Notebook/Protein_Engineering

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{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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