User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/10/16

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Revision as of 15:01, 16 October 2012

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Entry title

Zinc protoporphyrin IX synthesis

Objectives

The Zinc Protoporphyrin IX produced in previous labs was not pure enough for further studies, therefore it was synthesized again. The values of the reactants were doubled to force the reaction toward the products and completion.

Procedure1

  1. 20ml of DMF (solvent) was brought to boiling point under a condenser.
  2. 0.2009g of protoporphyrin IX was added to the solvent and the mixture was allowed to boil for 20min.
  3. 0.0458g of zinc acetate was added to the boiling mixture and the reaction was allowed to run over the night.

Procedure2


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