User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/10/16

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Zinc protoporphyrin IX synthesis


The Zinc Protoporphyrin IX produced in previous labs was not pure enough for further studies, therefore it was synthesized again. The values of the reactants were doubled to force the reaction toward the products and completion.


  1. 20ml of DMF (solvent) was brought to boiling point.
  2. 0.2009g of protoporphyrin IX was added to the solvent and the mixture was allowed to boil under a condenser for 20min.
  3. 0.0458g of zinc acetate was added to the boiling mixture and the reaction was allowed to complete at the boiling point for 30min.

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