User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/11/27: Difference between revisions
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#After 20min, the reaction was still not complete therefore 0.163g of copper sulphate was added to the reaction again and the reaction was allowed to run for 20 more minutes. | #After 20min, the reaction was still not complete therefore 0.163g of copper sulphate was added to the reaction again and the reaction was allowed to run for 20 more minutes. | ||
#After this period of time, the product was tested in fluorescence spectrometry again and because there was no peaks of light emission at 640nm, it was concluded that the reaction was complete. | #After this period of time, the product was tested in fluorescence spectrometry again and because there was no peaks of light emission at 640nm, it was concluded that the reaction was complete. | ||
#The product of this reaction was added to 250ml of cold distilled water and was stored in refrigerator at 4 degree C | #The product of this reaction was added to 250ml of cold distilled water and was stored in refrigerator at 4 degree C. | ||
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Revision as of 13:20, 29 November 2012
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Entry titleSynthesis of copper protoporphyrin IX ObjectiveCopper sulphate was reacted with protoporphyrin IX to produce copper protoporphyrin Procedure
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