User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/22

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(Objective 1)
(Procedure 1)
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==Procedure 1==  
==Procedure 1==  
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# 5ml of LB was added to two set of sterile tubes by 10 ml pipets
+
# 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets
# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube
# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube
-
#  
+
# The frozen cells in glycerol were taken out of the -80 degree C fridge
 +
# Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance
 +
# PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance
 +
# The tubes were places in the incubator/shaker at 37 degree C for 24h
 +
 
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==Objective 2==   
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Revision as of 09:20, 29 January 2013

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Entry title

Culturing antibiotic resistant bacteria producing myoglobin and hemoglobin

Objective 1

Ampicillin and Kenamycin were the antibiotics used for culturing

Procedure 1

  1. 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets
  2. 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube
  3. The frozen cells in glycerol were taken out of the -80 degree C fridge
  4. Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance
  5. PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance
  6. The tubes were places in the incubator/shaker at 37 degree C for 24h

Objective 2


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