User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/22: Difference between revisions
From OpenWetWare
| (5 intermediate revisions by the same user not shown) | |||
| Line 13: | Line 13: | ||
==Procedure 1== | ==Procedure 1== | ||
# 5ml of LB was added to two set of sterile tubes by 10 ml pipets | # 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets | ||
# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube | # 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube | ||
# | # The frozen cells in glycerol were taken out of the -80 degree C fridge | ||
# Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance | |||
# PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance | |||
# The tubes were placed in the incubator/shaker at 37 degree C for 24h | |||
==Objective 2== | |||
Lyophilization of 0.0058g Myoglobin, 20ml PH 7 phosphate, 0.4961 mg KCL sample | |||
==Procedure 2== | |||
# The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze it | |||
# When all the liquid of the sample was solidified, the sample were place under vacuum to make the ice to sublimate into vapor water directly and to leave the sample | |||
# The vacuum was allowed to run over night for 24h | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 06:56, 5 February 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleCulturing antibiotic resistant bacteria producing myoglobin and hemoglobin Objective 1Ampicillin and Kenamycin were the antibiotics used for culturing Procedure 1
Objective 2Lyophilization of 0.0058g Myoglobin, 20ml PH 7 phosphate, 0.4961 mg KCL sample Procedure 2
| |
