User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/22
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==Procedure 1== | ==Procedure 1== | ||
| - | # 5ml of LB was added to two set of sterile tubes by 10 ml pipets | + | # 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets |
# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube | # 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube | ||
| - | # | + | # The frozen cells in glycerol were taken out of the -80 degree C fridge |
| + | # Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance | ||
| + | # PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance | ||
| + | # The tubes were places in the incubator/shaker at 37 degree C for 24h | ||
| + | |||
| + | ==Objective 2== | ||
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Revision as of 09:20, 29 January 2013
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Entry titleCulturing antibiotic resistant bacteria producing myoglobin and hemoglobin Objective 1Ampicillin and Kenamycin were the antibiotics used for culturing Procedure 1
Objective 2 | |
