User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/22 - Revision history

NYOUSHA Yousefi: /* Procedure 1 */

NYOUSHA Yousefi — Tue, 05 Feb 2013 13:56:13 GMT

Procedure 1

←Older revision		Revision as of 13:56, 5 February 2013
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	# Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance		# Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance
	# PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance		# PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance
-	# The tubes were ~~places~~ in the incubator/shaker at 37 degree C for 24h	+	# The tubes were placed in the incubator/shaker at 37 degree C for 24h

	==Objective 2==		==Objective 2==

NYOUSHA Yousefi — Tue, 29 Jan 2013 13:29:32 GMT

Procedure 2

←Older revision		Revision as of 13:29, 29 January 2013
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	==Procedure 2==		==Procedure 2==
	# The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze it		# The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze it
-	# When all the liquid of the sample was solidified, the ~~samples~~ were place under vacuum to make the ice to sublimate into vapor water directly	+	# When all the liquid of the sample was solidified, the sample were place under vacuum to make the ice to sublimate into vapor water directly and to leave the sample
	# The vacuum was allowed to run over night for 24h		# The vacuum was allowed to run over night for 24h
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NYOUSHA Yousefi — Tue, 29 Jan 2013 13:28:42 GMT

Procedure 2

←Older revision		Revision as of 13:28, 29 January 2013
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	==Procedure 2==		==Procedure 2==
-	# The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze ~~them~~	+	# The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze it
	# When all the liquid of the sample was solidified, the samples were place under vacuum to make the ice to sublimate into vapor water directly		# When all the liquid of the sample was solidified, the samples were place under vacuum to make the ice to sublimate into vapor water directly
	# The vacuum was allowed to run over night for 24h		# The vacuum was allowed to run over night for 24h

NYOUSHA Yousefi — Tue, 29 Jan 2013 13:27:12 GMT

Procedure 2

←Older revision		Revision as of 13:27, 29 January 2013
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	==Procedure 2==		==Procedure 2==
		+	# The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze them
		+	# When all the liquid of the sample was solidified, the samples were place under vacuum to make the ice to sublimate into vapor water directly
		+	# The vacuum was allowed to run over night for 24h
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	\|}		\|}

	__NOTOC__		__NOTOC__

NYOUSHA Yousefi — Tue, 29 Jan 2013 13:22:33 GMT

Objective 2

←Older revision		Revision as of 13:22, 29 January 2013
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	==Objective 2==		==Objective 2==
		+	Lyophilization of 0.0058g Myoglobin, 20ml PH 7 phosphate, 0.4961 mg KCL sample

		+	==Procedure 2==
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	\|}		\|}

	__NOTOC__		__NOTOC__

NYOUSHA Yousefi — Tue, 29 Jan 2013 13:20:21 GMT

Procedure 1

←Older revision		Revision as of 13:20, 29 January 2013
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	==Procedure 1==		==Procedure 1==
-	# 5ml of LB was added to two set of sterile tubes by 10 ml pipets	+	# 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets
	# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube		# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube
-	#	+	# The frozen cells in glycerol were taken out of the -80 degree C fridge
		+	# Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance
		+	# PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance
		+	# The tubes were places in the incubator/shaker at 37 degree C for 24h
		+
		+	==Objective 2==

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NYOUSHA Yousefi — Tue, 29 Jan 2013 13:11:52 GMT

Objective 1

←Older revision		Revision as of 13:11, 29 January 2013
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	==Objective 1==		==Objective 1==
-	~~Ampicilin~~ and Kenamycin were the antibiotics used for culturing	+	Ampicillin and Kenamycin were the antibiotics used for culturing

	==Procedure 1==		==Procedure 1==

NYOUSHA Yousefi — Tue, 29 Jan 2013 13:11:40 GMT

Procedure 1

←Older revision		Revision as of 13:11, 29 January 2013
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	==Procedure 1==		==Procedure 1==
		+	# 5ml of LB was added to two set of sterile tubes by 10 ml pipets
		+	# 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube
		+	#

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NYOUSHA Yousefi — Tue, 29 Jan 2013 13:08:19 GMT

Objective 1

←Older revision		Revision as of 13:08, 29 January 2013
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	==Objective 1==		==Objective 1==
		+	Ampicilin and Kenamycin were the antibiotics used for culturing

		+	==Procedure 1==

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NYOUSHA Yousefi — Tue, 29 Jan 2013 13:05:17 GMT

Entry title

←Older revision		Revision as of 13:05, 29 January 2013
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	==Entry title==		==Entry title==
-	* Insert content here...	+	Culturing antibiotic resistant bacteria producing myoglobin and hemoglobin
		+
		+	==Objective 1==