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Entry title
Culturing antibiotic resistant bacteria producing myoglobin and hemoglobin
Objective 1
Ampicillin and Kenamycin were the antibiotics used for culturing
Procedure 1
- 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets
- 5ul of Ampicillin was added to one tube and Kenamycin was added to the other tube
- The frozen cells in glycerol were taken out of the -80 degree C fridge
- Bacteria expressing Myoglobin were added to the media tube containing Ampicillin because it has Ampicillin resistance
- PQE-80 bacteria were added to media tube containing Kenamycin becasue it has Kenamycin resistance
- The tubes were places in the incubator/shaker at 37 degree C for 24h
Objective 2
Lyophilization of 0.0058g Myoglobin, 20ml PH 7 phosphate, 0.4961 mg KCL sample
Procedure 2
- The tube of the sample was placed in liquid nitrogen for 15 min to completely freeze it
- When all the liquid of the sample was solidified, the samples were place under vacuum to make the ice to sublimate into vapor water directly
- The vacuum was allowed to run over night for 24h
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