User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/29

(Difference between revisions)

Revision as of 09:48, 5 February 2013

Project name

Preparing protein solutions

Eight different protein solutions containing were prepared for lyophilization

0.2g D-manitol was added to four set of new tubes and 0.2g P-sorbitol was added to another set of four tubes, each tube recieved 0.2g of sugar
0.1g of Polyethylene Glycol (PEG), M.W. 20,000 was added to all tubes
10ml of four set of buffers including phosphate, tris, citrate, and acetate were added to tubes in an order that each four set of tubes received only one of these buffers
The tubes were vortexed to dissolve PEG into the buffer and sugar solution
Finally the tubes were placed in -80 degree C to freeze and then they were placed in the vacuum for lyophilization

@@ Line 13: / Line 13: @@
 ==Procedure==
+# 0.2g D-manitol was added to four set of new tubes and 0.2g P-sorbitol was added to another set of four tubes, each tube recieved 0.2g of sugar
+# 0.1g of Polyethylene Glycol (PEG), M.W. 20,000 was added to all tubes
+# 10ml of four set of buffers including phosphate, tris, citrate, and acetate were added to tubes in an order that each four set of tubes received only one of these buffers
+# The tubes were vortexed to dissolve PEG into the buffer and sugar solution
+# Finally the tubes were placed in -80 degree C to freeze and then they were placed in the vacuum for lyophilization
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