User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/02/05: Difference between revisions
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==Procedure 1== | ==Procedure 1== | ||
# | # Column chromatogrophy was rinsed with 25ml of distilled water and 25ml of new buffer solution containing 0.5g PEG, 1g manitol,and 50ml of phosphate buffer, pH 8 | ||
# 2.5ml of WT Hb and 2.5ml of the new buffer were added to the column and this 5ml was collected in a new tube (2% manitol, 1% PEG, WT Hb, pH 7 phosphate) | # 2.5ml of WT Hb and 2.5ml of the new buffer were added to the column and this 5ml was collected in a new tube (2% manitol, 1% PEG, WT Hb, pH 7 phosphate) | ||
Revision as of 17:30, 18 February 2013
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TitleHb buffer exchange ObjectiveThe wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml phosphate pH 7 Procedure 1
Objective 2Extracting bacteria cells Procedure 2
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