User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/02/05: Difference between revisions
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The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8 | The wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8 | ||
==Procedure== | ==Procedure 1== | ||
# column chromatogrophy was rinsed with 25ml of distilled water and 25ml of new buffer solution containing 0.5g PEG, 1g manitol,and 50ml of phosphate buffer, pH 8 | |||
# 2.5ml of WT Hb and 2.5ml of the new buffer were added to the column and this 5ml was collected in a new tube (2% manitol, 1% PEG, WT Hb, pH 7 phosphate) | |||
==Procedure 2== | |||
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TitleHb buffer exchange ObjectiveThe wild type Hb's buffer was exchanged to a new buffer containing 1g manitol, o.5g PEG, and 50ml Tris pH 8 Procedure 1
Procedure 2 | |
