User:Nader A. Khamis/Notebook/Experimental Biological Chemistry AU Fall 2011/2011/09/01: Difference between revisions

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==Description==
==Description==
# Approximately 9 mL of ethanol and 1 mL of distilled H<sub>2</sub>O were placed in a 10 mL graduated cylinder to prepare  a 90% ethanol solution.
# Approximately 1g of Zein was placed into an erlenmeyer flask.
#Approximately 240μl of glycerol was carefully inserted into the erlenmeyer flask.
#Approximately 8.7 mL of ethanol was pipetted into the erlenmayer flask and the mixture was mixed using a stir bar.
# 5g of the mixture was transfered to a 9cm diameter Petri dish.
# The cover of the petri dish was removed and the petri dish was placed into the oven for 24hours. The oven temperature was 40°C.


==Data==
==Data==

Revision as of 13:35, 9 December 2011

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Objective

Description

Data

  • A solution of Zein (10% w/v) along with Ethanol (90%v/v) and Glycerol (30% on Zein weight basis) was prepared by using the following procedure.
  • Approximately 9 mL Ethanol and 1 mL distilled water were added to a 10 mL graduated cylinder.
 Calculation: (9ml/10ml)*100%= 90% v/v Ethanol
  • To prepare 10%w/v Zein: 0.1 = x grams of zein / 10ml of solution
                          x= 1g of Zein.
  • To prepare Glycerol (30% on Zein weight basis): 0.3*(1g of Zein)= 0.3g of Glycerol
  • The density of Glycerol equals 0.261g/cm3.
  • 1.261g/ml=0.3g/x ml of Glycerol.
x = 0.24ml of Glycerol

Notes

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