User:Nader A. Khamis/Notebook/Experimental Biological Chemistry AU Fall 2011/2011/09/02: Difference between revisions

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==Objective==
==Objective==
* Synthesis of Bovine Serum Albumen Conjugated Gold Nanoparticles. This is a repetition of the experiment performed on August 31<sup>st</sup>, 2011.


==Description==
==Description==

Revision as of 13:37, 9 December 2011

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Objective

Description

  1. Prepare 10ml aqueous mixtures of BSA (1.5μM)and HAuCl4 (0.25mM) at room temperature.
  2. Prepare 10ml mixture of BSA (1.5μM)and HAuCl4 (0.25mM) using 50mM of Acetate as the buffer. The pH of the buffer was 4.3.
  3. Place each reaction mixture in an 80°C water bath.
  4. Obtain UV-Vis Spectra of gold colloidal suspensions in the wavelength range of 200-800nm at 30 minute intervals.

Data

2.5 mM of HAuCl4 and 15 μM of BSA were the two stock solutions used.

To prepare BSA (1.5μM)and HAuCl4 (0.25mM), the equation M1V1=M2V2 was used.

  • Calculation

2.5mM*V1 = 0.25mM*10ml

V1= 1ml of HAuCl4


0.015mM*V1 = 0.0015 mM*10ml

V1= 1ml of BSA


  • 1ml of HAuCl and 1ml of BSA were placed in each of the two test tubes.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. .


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