User:Nadiezda Fernandez-Oropeza/Notebook/Notebook/2010/10/11

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

General Lab procedures

  • This entry contains details on handling chemicals and the operation of some lab instruments.

For the preparation of a solution

First, get all the materials necessary on the work bench. This will include: electronic scale, chemicals, spatulas and tweezers, gloves, caped tubes, racks, weighing paper and boats, etc.

  • Electronic scale

First it needs to be leveled out with respect to the work bench. To assure it is, take a look at the bubble indicator. If it is not centered, move the knobs located in the four bottom corners of the scale. Unfortunately, to operate the four knobs is not an easy task and the best way to operate is to guide the bubble in the two possible inclination planes.

Second, the scale is very sensitive. Therefore, when taking measurements the two side-doors need to be closed and you need to avoid any harsh moves around the scale.

  • Spatulas and tweezers

First, clean them following the protocol. (Check the section “Cleaning” in the following link:[[1]])

Second, let them sit on paper towels on top of the work bench. Also, they should remain covered to avoid contamination.

  • Chemicals

Do the measurement of one chemical at a time. Choose which one you are going to work with and stick to it until you finish.

NOTHING can be placed inside the stock solution container. This way we avoid possible contamination. Thus, reasonable amounts of the chemical need to be poured in separate weighing boats.

  • Weighing boats and papers

To see how to handle these two, check the section How to properly weigh a sample in the following link:[[2]]

Moreover, try to hold them from the edges, as far as possible from where the sample is going to be placed.

Transferring the sample

Once you have all the needed chemicals they need to be placed on a tube.

  • How to pour the chemicals from the weighing boat to the tube

Carefully remove the weighing boat from the scale and form a subtle cone shape with it. Place the tip vertically inside the tube and then with the help of a spatula, push the entire chemical down until most of it is poured.

If you are using the wax paper the procedure is similar, with the advantage that the wax paper has already a crack and serves as a canal directed to the tube.

Cleaning

  • [Proper disposal of Acids and Bases]
  • Everything needs to be placed back in their storage locations and the work bench and surroundings need to be cleaned with soap solution.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Nadiezda_Fernandez-Oropeza/Notebook/Notebook/2010/10/11&oldid=462978"