User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/02/26: Difference between revisions
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===Ag:BSA Nanofibers: Results=== | ==Objective== | ||
*Make 5 sets of PLA scaffolds: untreated PLA scaffolds, nanofiber-coated scaffolds by Brute-force method, biomineralization method of nanofiber formation with scaffolds, polydopamine-coated scaffolds, and polydopamine nanofiber-coated scaffolds | |||
*Collect result data from last lab | |||
==Methods== | |||
*Each set consisted of 3 test tubes, each containing 4 stacked PLA scaffolds. | |||
*All scaffolds were Autoclaved at then end of each procedure. | |||
===Plain scaffold=== | |||
*The scaffolds were submerged in water | |||
===Brute Force method=== | |||
*100:1 Au:Myoglobin nanofibers were made based on previous biomineralization protocol | |||
*The nanofibers were then place onto the scaffolds and allowed to dry | |||
*The dry nanofiber-coated scaffolds were then submerged in water | |||
===Old method + scaffolds=== | |||
*100:1 Au:Myoglobin nanofibers were made following previously used protocol, but with the PLA scaffolds in the test tubes | |||
===Polydopamine-coated scaffolds=== | |||
*Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour | |||
*Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours | |||
*Rinse with deionized water and allow scaffold to dry overnight | |||
*Scaffolds submerged in water | |||
===Polydopamine Nanofiber-coated scaffolds=== | |||
*Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour | |||
*Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours | |||
*Rinse with deionized water and allow scaffold to dry overnight | |||
*Scaffolds submerged in water | |||
*Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution | |||
==Results== | |||
===Ag:BSA Nanofibers: Results from 02/25/2014=== | |||
*Set 1 (All ratios, room temperature) | *Set 1 (All ratios, room temperature) | ||
[[Image:30 original.jpg|300px]] | [[Image:30 original.jpg|300px]] |
Revision as of 14:19, 20 March 2014
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Objective
Methods
Plain scaffold
Brute Force method
Old method + scaffolds
Polydopamine-coated scaffolds
Polydopamine Nanofiber-coated scaffolds
ResultsAg:BSA Nanofibers: Results from 02/25/2014
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