User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/03/26

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Biomaterials Design Lab Main project page
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Objective

Ag:BSA NPs from 03/26/2014 UVvis of Ag:BSA NPs from 03/19/2014 UVvis of Ag:BSA NPs from 03/26/2014 Put scaffolds into cell culture

Methods for cells

  • Cells were cultured in petri dishes but transferred to T75 cell culture flasks (with red cap) at the end of protocol
  • 5 flasks were labeled such that each flask will contain scaffolds from each type of treatment, 9 scaffolds in each flask
  • Cells were placed in each flask and incubated

Results

  • All samples were a cloudy white solution, but had a yellow pellet in the base of the test tube which increased in size with decreasing concentration




Measurements: UV-vis Ag:BSA NPs

  • Samples from 03/19/2014, 1uL Au Added, after being vortexed

Image:2014_0326_abs_Ag_BSA_0319_vortexed.PNG

  • Samples from 03/19/2014, 1mL Au Added, after being vortexed

Image:2014_0326_abs_Ag_BSA_0319_vortexed_1mL.PNG

  • Samples from 03/25/2014

Image:2014_0326_abs_Ag_BSA_0325.PNG

  • Samples from 03/25/2014, after being diluted

Image:2014_0326_abs_Ag_BSA_0325_diluted.PNG

Cell results

  • Cells became contaminated; cultures were discarded



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