User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/03/26

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(Autocreate 2014/03/26 Entry for User:Nancy_T._Miles/Notebook/CHEM_572_Spr_2014)
Current revision (10:34, 7 May 2014) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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* Insert content here...
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==Objective==
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Ag:BSA NPs from 03/26/2014
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UVvis of Ag:BSA NPs from 03/19/2014
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UVvis of Ag:BSA NPs from 03/26/2014
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Put scaffolds into cell culture
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==Methods for cells==
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*Cells were cultured in petri dishes but transferred to T75 cell culture flasks (with red cap) at the end of protocol
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*5 flasks were labeled such that each flask will contain scaffolds from each type of treatment, 9 scaffolds in each flask
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*Cells were placed in each flask and incubated
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==Results==
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*All samples were a cloudy white solution, but had a yellow pellet in the base of the test tube which increased in size with decreasing concentration
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[[Image:0326_70,_1uL_Au.jpg|300px]]  [[Image:0326_90,_1uL_Au.jpg|300px]]<br.>
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[[Image:0326_110,_1uL_Au.jpg|300px]]  [[Image:0326_130,_1uL_Au.jpg|300px]]<br.>
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[[Image:0326_150,_1uL_Au.jpg|300px]]  [[Image:0326_170,_1uL_Au.jpg|300px]]<br.>
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===Measurements: UV-vis Ag:BSA NPs===
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*Samples from 03/19/2014, 1uL Au Added, after being vortexed
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[[Image:2014_0326_abs_Ag_BSA_0319_vortexed.PNG]]
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*Samples from 03/19/2014, 1mL Au Added, after being vortexed
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[[Image:2014_0326_abs_Ag_BSA_0319_vortexed_1mL.PNG]]
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*Samples from 03/25/2014
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[[Image:2014_0326_abs_Ag_BSA_0325.PNG]]
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*Samples from 03/25/2014, after being diluted
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[[Image:2014_0326_abs_Ag_BSA_0325_diluted.PNG]]
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===Cell results===
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*Cells became contaminated; cultures were discarded

Current revision

Biomaterials Design Lab Main project page
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Objective

Ag:BSA NPs from 03/26/2014 UVvis of Ag:BSA NPs from 03/19/2014 UVvis of Ag:BSA NPs from 03/26/2014 Put scaffolds into cell culture

Methods for cells

  • Cells were cultured in petri dishes but transferred to T75 cell culture flasks (with red cap) at the end of protocol
  • 5 flasks were labeled such that each flask will contain scaffolds from each type of treatment, 9 scaffolds in each flask
  • Cells were placed in each flask and incubated

Results

  • All samples were a cloudy white solution, but had a yellow pellet in the base of the test tube which increased in size with decreasing concentration




Measurements: UV-vis Ag:BSA NPs

  • Samples from 03/19/2014, 1uL Au Added, after being vortexed

Image:2014_0326_abs_Ag_BSA_0319_vortexed.PNG

  • Samples from 03/19/2014, 1mL Au Added, after being vortexed

Image:2014_0326_abs_Ag_BSA_0319_vortexed_1mL.PNG

  • Samples from 03/25/2014

Image:2014_0326_abs_Ag_BSA_0325.PNG

  • Samples from 03/25/2014, after being diluted

Image:2014_0326_abs_Ag_BSA_0325_diluted.PNG

Cell results

  • Cells became contaminated; cultures were discarded



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