User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/03/26: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | |||
* | ==Objective== | ||
Ag:BSA NPs from 03/26/2014 | |||
UVvis of Ag:BSA NPs from 03/19/2014 | |||
UVvis of Ag:BSA NPs from 03/26/2014 | |||
Put scaffolds into cell culture | |||
==Methods for cells== | |||
*Cells were cultured in petri dishes but transferred to T75 cell culture flasks (with red cap) at the end of protocol | |||
*5 flasks were labeled such that each flask will contain scaffolds from each type of treatment, 9 scaffolds in each flask | |||
*Cells were placed in each flask and incubated | |||
==Results== | |||
*All samples were a cloudy white solution, but had a yellow pellet in the base of the test tube which increased in size with decreasing concentration | |||
[[Image:0326_70,_1uL_Au.jpg|300px]] [[Image:0326_90,_1uL_Au.jpg|300px]]<br.> | |||
[[Image:0326_110,_1uL_Au.jpg|300px]] [[Image:0326_130,_1uL_Au.jpg|300px]]<br.> | |||
[[Image:0326_150,_1uL_Au.jpg|300px]] [[Image:0326_170,_1uL_Au.jpg|300px]]<br.> | |||
===Measurements: UV-vis Ag:BSA NPs=== | |||
*Samples from 03/19/2014, 1uL Au Added, after being vortexed | |||
[[Image:2014_0326_abs_Ag_BSA_0319_vortexed.PNG]] | |||
*Samples from 03/19/2014, 1mL Au Added, after being vortexed | |||
[[Image:2014_0326_abs_Ag_BSA_0319_vortexed_1mL.PNG]] | |||
*Samples from 03/25/2014 | |||
[[Image:2014_0326_abs_Ag_BSA_0325.PNG]] | |||
*Samples from 03/25/2014, after being diluted | |||
[[Image:2014_0326_abs_Ag_BSA_0325_diluted.PNG]] | |||
===Cell results=== | |||
*Cells became contaminated; cultures were discarded | |||
Revision as of 08:34, 7 May 2014
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAg:BSA NPs from 03/26/2014 UVvis of Ag:BSA NPs from 03/19/2014 UVvis of Ag:BSA NPs from 03/26/2014 Put scaffolds into cell culture Methods for cells
Results
Measurements: UV-vis Ag:BSA NPs
Cell results
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