User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/04/08

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(Autocreate 2014/04/08 Entry for User:Nancy_T._Miles/Notebook/CHEM_572_Spr_2014)
Current revision (10:43, 7 May 2014) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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* Insert content here...
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==Objectives==
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*Centrifuge 2nd procedure Ag:BSA NPs
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*UVvis of Ag:BSA NPs from last time
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*Put dry-autoclaved scaffolds into bacteria
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*Make Au nanofibers and print scaffolds (12) for brute force
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==Sample Prep: Nanofibers for Brute Force==
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[[Image:2014_0211_myo_bsa_NPs.PNG]]
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*Solutions:
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**Au: 0.011g in 10mL to make 2.793mM solution
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**Myoglobin: 0.011g in 10mL to make 0.0621mM solution
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===Brute Force Nanofiber-coated Scaffolds===
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*4 PLA scaffolds printed for each test tube
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*Gold:Myoglobin (100:1) nanofibers synthesized by above method
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*Nanofibers dried onto PLA scaffolds
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*Scaffolds submerged in water
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*Autoclaved
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==Results: UV-vis==
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[[Image:2014 0408 abs AgBSA NPs.PNG]]
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*Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm
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==Sample Prep: Scaffolds in Bacteria==
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*E. coli bacteria culture were prepared
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*Bacteria culture media was prepared and placed in 5 small test tubes, each tube for each type of scaffold treatment
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*E. coli were placed in test tubes
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*3 Dry-autoclaved scaffolds submerged in each test tube of bacteria solution
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*Test tubes with cells and scaffolds were placed in shaker until next class
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Current revision

Biomaterials Design Lab Main project page
Previous entry      Next entry

Objectives

  • Centrifuge 2nd procedure Ag:BSA NPs
  • UVvis of Ag:BSA NPs from last time
  • Put dry-autoclaved scaffolds into bacteria
  • Make Au nanofibers and print scaffolds (12) for brute force

Sample Prep: Nanofibers for Brute Force

Image:2014_0211_myo_bsa_NPs.PNG

  • Solutions:
    • Au: 0.011g in 10mL to make 2.793mM solution
    • Myoglobin: 0.011g in 10mL to make 0.0621mM solution

Brute Force Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above method
  • Nanofibers dried onto PLA scaffolds
  • Scaffolds submerged in water
  • Autoclaved

Results: UV-vis

Image:2014 0408 abs AgBSA NPs.PNG

  • Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm

Sample Prep: Scaffolds in Bacteria

  • E. coli bacteria culture were prepared
  • Bacteria culture media was prepared and placed in 5 small test tubes, each tube for each type of scaffold treatment
  • E. coli were placed in test tubes
  • 3 Dry-autoclaved scaffolds submerged in each test tube of bacteria solution
  • Test tubes with cells and scaffolds were placed in shaker until next class



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