User:Nathan H. Kipniss/Notebook/20.109 Final Project
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In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes. | In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes. | ||
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| + | [Media:S12_M2D7_TR-Orange.txt|Raw Titration Data] | ||
[Image:Plots,_fitted_Matlab.jpg] | [Image:Plots,_fitted_Matlab.jpg] | ||
Revision as of 12:17, 1 May 2012
Elucidating the function of D24H Inverse Pericam mutant
Background
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.
[Media:S12_M2D7_TR-Orange.txt|Raw Titration Data]
[Image:Plots,_fitted_Matlab.jpg]
Ideas
-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work)
- consider looking at calmodulin only.
