User:Nathan H. Kipniss/Notebook/20.109 Final Project

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(Background)
(Background)
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==Background==
==Background==
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.
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|[[Media:S12_M2D7_TR-Orange.txt]]
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[Media:S12_M2D7_TR-Orange.txt|Raw Titration Data]
[Image:Plots,_fitted_Matlab.jpg]
[Image:Plots,_fitted_Matlab.jpg]

Revision as of 12:17, 1 May 2012

Elucidating the function of D24H Inverse Pericam mutant

Background

In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.

[Media:S12_M2D7_TR-Orange.txt|Raw Titration Data]

[Image:Plots,_fitted_Matlab.jpg]

Ideas

-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work)

- consider looking at calmodulin only.

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