User:Nathan H. Kipniss/Notebook/20.109 Final Project

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(Background)
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- consider looking at calmodulin only.
- consider looking at calmodulin only.
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==Papers Currently being read==
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Grossman,M et al. âœAchieving broad molecular insights into dynamic protein interactions by integrated structural-kinetic approaches. Current opinion in structural biology 21.5 (2011): 678-85. Web. 1 Mar. 2012.

Revision as of 12:22, 1 May 2012

Contents

Elucidating the function of D24H Inverse Pericam mutant

Background

In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.

[Media:S12_M2D7_TR-Orange.txt]

Fitted Matlab binding curves
Fitted Matlab binding curves

Ideas

-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work)

- consider looking at calmodulin only.

Papers Currently being read

Grossman,M et al. âœAchieving broad molecular insights into dynamic protein interactions by integrated structural-kinetic approaches. Current opinion in structural biology 21.5 (2011): 678-85. Web. 1 Mar. 2012.

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