User:Nathan H. Kipniss/Notebook/20.109 Final Project
Elucidating the function of D24H Inverse Pericam mutant
Background
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.
[Media:S12_M2D7_TR-Orange.txt]
Ideas
-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work)
- consider looking at calmodulin only.
Papers Currently being read
Grossman,M et al. Achieving broad molecular insights into dynamic protein interactions by integrated structural-kinetic approaches. Current opinion in structural biology 21.5 (2011): 678-85. Web. 1 Mar. 2012. Scapin, G. Structural Biology and Drug Discovery. Current Pharmaceutical Design. 2006
